Biol. Pharm. Bull. 29(1) 187—189 (2006)

mortality in compromised hosts. Fukazawa et al. designed a slide agglutination scheme which uses factor sera, whereby it is possible to make a rapid and accurate identification of medically important Candida species as a commercial available factor serum kit (Candida Check). With the objective to clarify the antigenicity of C. albicans cells grown in a patient’s body, we used the body temperature (high temperature, 37 °C) for the Candida cell cultivation. The results showed that the mannans prepared from all the strains of C. albicans serotypes A and B cultured at high temperature (37 °C) in yeast extract-added Sabouraud liquid medium (YSLM) composed of 0.5% (w/v) yeast extract, 1% (w/v) peptone, and 4% (w/v) glucose and in 500 mM galactoseadded yeast nitrogen base medium (YNB-Gal) lost their reactivity against the factor sera 5 and 6 in the ‘Candida Check’ and contained neither a phosphate group nor a b1,2-linked mannopyranose unit compared to that from the cells cultured under conventional conditions (27 °C) in the medium. It is known that Candida glabrata infections often result in high mobidity and mortality in immunocompromised hospitalized patients. The serological behavior of the pathogenic C. glabrata is that the determinants of Candida antigenic factors 1, 4, 6, and 34 have been detected on the cell surface mannan of the C. glabrata. The structure of the mannan of C. glabrata IFO 0622 strain cultured at 27 °C has already been reported. In this paper, we used body temperature (high temperatures, 37 °C or 36 °C) for the cultivation condition in order to clarify the entity of C. glabrata strain cells in vivo and compared the antigenicity, namely the mannan structure, of the strain cells cultured at 27 °C, 37 °C (or 36 °C), and 37—27 °C in the two media, YSLM and BACTEC fungal medium.